Recombinant protein expression service

Expression vector construction; transient transfection in eukaryotic cells (CHO/293); prokaryotic and yeast expression.

Development of monoclonal cell lines

Vector construction, and the exogenous gene was introduced into CHO/HEK293 cells by transfection, and the resistance gene was screened. The single cell printing system and monoclonal imaging system were combined to screen the monoclonal cell line with stable and high-throughput expression of the target protein.

Drug registration service

We provide services for drug registration, regulatory and process consulting, IND applications for innovative drugs, NDA submissions for new drug approvals, and preparation and submission of supplementary application documents, supporting pharmaceutical R&D and manufacturing companies in obtaining regulatory approvals.

Cell expansion and culture process

We can provide cell process development from shake flask to 200L scale, 2-10L multi-type cell culture pilot bioreactors, WAVE bioreactor culture, and 200L disposable bioreactor culture process.

Rotein purification process

Protein purification process

We can provide a variety of protein purification technology services such as large-scale protein purification, fusion tag protein purification, and tag-free protein purification

Quality analysis and validation

Lentiviral packaging service

Lentivirus vectors are tool vectors developed based on HIV-1 and using the herpes virus VSVG coat protein. Lentiviruses have a wide spectrum of infection and can effectively infect both dividing and non-dividing cells. Once infected with host cells, the lentivirus uses reverse transcriptase to transcribe its RNA into double-stranded DNA, which is then permanently integrated into the chromosomes of the host cells to achieve long-term stable expression. Based on the genetic information and service needs provided by customers, Anergy develops solutions that meet customer requirements and constructs overexpression lentivirus vectors. The packaged virus particles can be used for cell experiments and animal experiments.

Slow virus packaging service

Lentiviral vectors are tool vectors based on HIV-1 and developed using the herpesvirus VSVG envelope protein. They have a broad host range and can efficiently infect both dividing and non-dividing cells. Once inside the host cell, the lentivirus uses reverse transcriptase to convert its RNA into double-stranded DNA, which then integrates permanently into the host genome, enabling long-term stable expression. Based on the customer’s gene information and specific requirements, Energy designs customized solutions to construct overexpression lentiviral vectors. The packaged viral particles can be used for both in vitro and in vivo experiments.

Lmmune cell preparation service

Immune cells refer to cells that participate in or are related to immune responses, including lymphocytes, dendritic cells, monocytes/macrophages, granulocytes, mast cells, etc. Common cells involved in immune cell therapy include: CIK cells, NK cells, DC-CIK cells, etc. Anage can customize and culture different types of cells according to customer needs, including natural killer cells (NK), lymphocytes (T), CAR-T, CAR-NK cells, etc.