NK Cell Cryopreservation Solution Instructions

1. Product Overview

NK cell cryopreservation fluid is a serum-free, xenobiotic-free, chemically defined, ready-to-use cryopreservation fluid suitable for cryopreservation of NK cells from peripheral blood and cord blood. It does not require complex programmed cooling or preparation and is easy to use.

2. Packing Specifications

Item No.: FNK-2403; Specification: 100mL/bottle;

3. Storage conditions and shelf-life

Storage conditions: 2-8℃, keep away from light; validity period: 12 months

4. Product performance indicators

Appearance: light yellow clear liquid; pH value: 6.8-7.4 Endotoxin:<0.25EU/ml Osmotic pressure: 1200-2400 mOSm/Kg; Mycoplasma: negative; Fungi and bacteria: negative

5. How to operate

Cryopreservation of cells:

1. Observe the cell status before freezing, count the cells, and determine the cell viability (greater than 90%).

2. Collect the cells to be frozen in a centrifuge tube and centrifuge at 1500 rpm, centrifuge 5 min and discard the supernatant.

3. Adjust the cell freezing density (1.0-10.0×10 7 cell/mL) as needed, and slowly add an appropriate volume of NK cell freezing solution stored at 4°C to resuspend the cells.

4. Aliquot the cell suspension into cryopreservation tubes, tighten the tube caps, and label them.

5. Place the cryovial in a programmed cooling box (pre-cooled at 4°C), and then place it in a-80°C refrigerator or directly in a-80°C refrigerator. If long-term freezing is required, transfer it to liquid nitrogen after 12 hours.

Cell Recovery:

1. Preheat a water bath to 37°C in advance.

2. Quickly thaw the cryopreserved cell vial in a 37°C water bath until only a few ice crystals remain.

3. Clean the tube with 75% alcohol, quickly transfer the cell suspension into the culture medium (no need to preheat) and mix thoroughly.

4. 1500 rpm, centrifuge immediately for 5 min, discard the supernatant, and add fresh culture medium preheated at room temperature.

5. As needed, cells of appropriate density are seeded into a suitable culture container and transferred to an incubator for culture.

6. Precautions

1. resuspending cells with this product, it is best to transfer to-80℃ for storage within 10 minutes.

2. Cryopreservation of cells in the logarithmic growth phase can help improve the survival rate of revived cells.

3. When cells are thawing, the cryopreserved tubes should be thawed quickly. The shorter the time, the less impact on the cells.

4. The cell cryopreservation tube must be completely sealed, otherwise it may explode during the cell recovery process.

5. This product contains DMSO. For some cells that are sensitive to DMSO, it is recommended to conduct a preliminary experiment first.